Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture

Abstract Kluyveromyces marxianus is a yeast that could be identified from kefir and can use broad range of substrates, such as glucose lactate, carbon sources. The lactate produced in culture substrate for K. . However, the complexity microbiota makes traits difficult to study. In this research, we focused on cultured with sole source. optimal growth released protein were determined under different pH conditions, LC–MS/MS-identified proteins associated tricarboxylic acid cycle, glycolysis pathway, cellular stress responses cells, indicating autolysis had occurred conditions. abundant glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) was cocrystallized other cell-free fraction, low transcription level GAP1 gene indicated abundance conditions dependent stability. These results suggest induces , releasing peptides. findings fundamental probiotic studies future.